Search results for "Action Potentials"

showing 10 items of 192 documents

Subchronic vortioxetine treatment -but not escitalopram- enhances pyramidal neuron activity in the rat prefrontal cortex.

2017

Abstract Vortioxetine (VOR) is a multimodal antidepressant drug. VOR is a 5-HT 3 -R, 5-HT 7 -R and 5-HT 1D -R antagonist, 5-HT 1B -R partial agonist, 5-HT 1A -R agonist, and serotonin transporter (SERT) inhibitor. VOR shows pro-cognitive activity in animal models and beneficial effects on cognitive dysfunction in major depressive patients. Here we compared the effects of 14-day treatments with VOR and escitalopram (ESC, selective serotonin reuptake inhibitor) on neuronal activity in the medial prefrontal cortex (mPFC). Ten groups of rats (5 standard, 5 depleted of 5-HT with p -chlorophenylalanine -pCPA-, used as model of cognitive impairment) were fed with control food or with two doses of …

0301 basic medicineAgonistMalegenetic structuresmedicine.drug_classSerotonin reuptake inhibitorAction PotentialsPrefrontal CortexPharmacologyCitalopramSulfidesPartial agonistPiperazines03 medical and health sciencesCellular and Molecular Neuroscience0302 clinical medicinemedicinePremovement neuronal activityAnimalsRats WistarSerotonin transporterPharmacologyVortioxetinebiologyPyramidal CellsAntagonistAntidepressive AgentsRats030104 developmental biologybiology.proteinAntidepressantVortioxetinesense organsPsychologyNeuroscience030217 neurology & neurosurgerySelective Serotonin Reuptake InhibitorsNeuropharmacology
researchProduct

Neuronal Excitability And Spontaneous Synaptic Transmission In The Entorhinal Cortex Of Bdnf Heterozygous Mice

2018

Abstract Brain Derived Neurotropic Factor (BDNF) is a neutrophic factor that is required for the normal neuronal development and function. BDNF is involved in regulation of synapses as well as neuronal excitability. Entorhinal Cortex (EC) is a key brain area involved in many physiological and pathological processes. In this study we investigated the effects of chronically reduced BDNF levels on layer 3 pyramidal neurons of EC. We aimed to assess the effects of reduced levels of BDNF on firing properties, spontaneous synaptic currents and excitation/inhibition balance from acute brain slices. Patch clamp recordings were obtained from pyramidal neurons of Entorhinal Cortex Layer 3. Findings o…

0301 basic medicineMalemedicine.medical_specialtyHeterozygoteAction potentialAction PotentialsNeurotransmissionInhibitory postsynaptic potentialSynaptic Transmission03 medical and health sciencesMice0302 clinical medicineInternal medicinemedicinePremovement neuronal activityAnimalsEntorhinal CortexPatch clampChemistryGeneral NeuroscienceSpontaneous synaptic transmissionBrain-Derived Neurotrophic FactorExcitatory Postsynaptic PotentialsEntorhinal cortex030104 developmental biologyEndocrinologyInhibitory Postsynaptic Potentialsnervous systemGene Knockdown TechniquesExcitatory postsynaptic potentialFemale030217 neurology & neurosurgery
researchProduct

Multimodal determinants of phase-locked dynamics across deep-superficial hippocampal sublayers during theta oscillations

2020

Theta oscillations play a major role in temporarily defining the hippocampal rate code by translating behavioral sequences into neuronal representations. However, mechanisms constraining phase timing and cell-type-specific phase preference are unknown. Here, we employ computational models tuned with evolutionary algorithms to evaluate phase preference of individual CA1 pyramidal cells recorded in mice and rats not engaged in any particular memory task. We applied unbiased and hypothesis-free approaches to identify effects of intrinsic and synaptic factors, as well as cell morphology, in determining phase preference. We found that perisomatic inhibition delivered by complementary populations…

0301 basic medicineMaleneural circuits.Patch-Clamp TechniquesGeneral Physics and AstronomyAction PotentialsHippocampal formationCell morphologySettore BIO/09 - Fisiologia0302 clinical medicineTheta Rhythmlcsh:ScienceBiophysical modelPhysicsNeurons0303 health sciencesComputational modelMultidisciplinaryBiología molecularPyramidal CellsQDynamics (mechanics)Theta oscillationsFemaleAlgorithmsScienceNeurocienciasModels NeurologicalPhase (waves)Mice TransgenicNeural circuitsGeneral Biochemistry Genetics and Molecular BiologyArticle03 medical and health sciencesGlutamatergicMemory taskAnimalsComputer SimulationRats WistarCA1 Region Hippocampal030304 developmental biologyGeneral ChemistryMice Inbred C57BLKinetics030104 developmental biologySynapseslcsh:QNeuroscience030217 neurology & neurosurgeryBiophysical models
researchProduct

Assessing the Impact of Single-Cell Stimulation on Local Networks in Rat Barrel Cortex—A Feasibility Study

2019

In contrast to the long-standing notion that the role of individual neurons in population activity is vanishingly small, recent studies have shown that electrical activation of only a single cortical neuron can have measurable effects on global brain state, movement, and perception. Although highly important for understanding how neuronal activity in cortex is orchestrated, the cellular and network mechanisms underlying this phenomenon are unresolved. Here, we first briefly review the current state of knowledge regarding the phenomenon of single-cell induced network modulation and discuss possible underpinnings. Secondly, we show proof of principle for an experimental approach to elucidate …

0301 basic medicinePatch-Clamp TechniquesComputer scienceCortical neuronPopulationAction PotentialsStimulationjuxtacellularCatalysisArticleInorganic ChemistryRats Sprague-Dawleylcsh:Chemistry03 medical and health sciences0302 clinical medicineCortex (anatomy)medicinePremovement neuronal activityAnimalsCell stimulationRats Long-EvansPhysical and Theoretical ChemistryRats WistareducationMolecular Biologylcsh:QH301-705.5SpectroscopyNeuronseducation.field_of_studyOrganic ChemistrynanostimulationGeneral MedicineSomatosensory CortexBarrel cortexComputer Science ApplicationsRatsElectrophysiologyin vivo030104 developmental biologymedicine.anatomical_structurelcsh:Biology (General)lcsh:QD1-999Feasibility Studiesbarrel cortexNeuronSingle-Cell AnalysisNeuroscience030217 neurology & neurosurgeryInternational Journal of Molecular Sciences
researchProduct

Focal Cortical Lesions Induce Bidirectional Changes in the Excitability of Fast Spiking and Non Fast Spiking Cortical Interneurons

2014

A physiological brain function requires neuronal networks to operate within a well-defined range of activity. Indeed, alterations in neuronal excitability have been associated with several pathological conditions, ranging from epilepsy to neuropsychiatric disorders. Changes in inhibitory transmission are known to play a key role in the development of hyperexcitability. However it is largely unknown whether specific interneuronal subpopulations contribute differentially to such pathological condition. In the present study we investigated functional alterations of inhibitory interneurons embedded in a hyperexcitable cortical circuit at the border of chronically induced focal lesions in mouse …

500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie570Neural NetworksPostsynaptic CurrentExcitotoxicity610lcsh:MedicineNeurophysiologyAction PotentialsNeural Homeostasis600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheitmedicine.disease_causeInhibitory postsynaptic potentialMiceEpilepsyInterneuronsmedicineBiological neural networkAnimalslcsh:ScienceVisual CortexCerebral CortexMembrane potentialMultidisciplinarymusculoskeletal neural and ocular physiologylcsh:RNeurotransmissionBiology and Life SciencesExcitatory Postsynaptic Potentialsmedicine.diseaseVisual cortexmedicine.anatomical_structurenervous systemCellular NeuroscienceExcitatory postsynaptic potentiallcsh:QNeuroscienceResearch ArticleNeurosciencePLoS ONE
researchProduct

Factors affecting basket catheter detection of real and phantom rotors in the atria: A computational study

2018

[EN] Anatomically based procedures to ablate atrial fibrillation (AF) are often successful in terminating paroxysmal AF. However, the ability to terminate persistent AF remains disappointing. New mechanistic approaches use multiple-electrode basket catheter mapping to localize and target AF drivers in the form of rotors but significant concerns remain about their accuracy. We aimed to evaluate how electrode-endocardium distance, far-field sources and inter-electrode distance affect the accuracy of localizing rotors. Sustained rotor activation of the atria was simulated numerically and mapped using a virtual basket catheter with varying electrode densities placed at different positions withi…

Ablation TechniquesNormalization propertyTime FactorsPhysiologymedicine.medical_treatmentAction Potentials02 engineering and technology030204 cardiovascular system & hematologylaw.invention0302 clinical medicineModelslawHeart RateMedicine and Health SciencesMapping cathetersCardiac Atrialcsh:QH301-705.5Numerical AnalysisEcologyRotor (electric)HeartAblationElectrophysiologyComputational Theory and Mathematicsmedicine.veinModeling and SimulationRotorsPhysical SciencesInferior Vena CavaEngineering and TechnologyAnatomyBasket catheterArrhythmiaInterpolationResearch ArticleBiotechnologyMaterials scienceCatheters0206 medical engineeringPhase (waves)CardiologyInferior vena cavaModels BiologicalMembrane PotentialImaging phantomVeinsTECNOLOGIA ELECTRONICA03 medical and health sciencesCellular and Molecular NeuroscienceAblation Techniques; Action Potentials; Atrial Fibrillation; Computational Biology; Computer Simulation; Heart Atria; Heart Conduction System; Heart Rate; Humans; Models Biological; Time FactorsHeart Conduction SystemPhase analysisGeneticsmedicineHumansComputer SimulationHeart AtriaMolecular BiologyEcology Evolution Behavior and SystematicsMechanical EngineeringComputational BiologyBiology and Life SciencesBiological020601 biomedical engineeringAtrial fibrillationInterpolationlcsh:Biology (General)Cardiovascular AnatomyBlood VesselsMedical Devices and EquipmentMathematicsBiomedical engineeringEndocardium
researchProduct

Rapid developmental switch in the mechanisms driving early cortical columnar networks

2006

The immature cerebral cortex self-organizes into local neuronal clusters long before it is activated by patterned sensory inputs. In the cortical anlage of newborn mammals, neurons coassemble through electrical or chemical synapses either spontaneously or by activation of transmitter-gated receptors. The neuronal network and the cellular mechanisms underlying this cortical self-organization process during early development are not completely understood. Here we show in an intact in vitro preparation of the immature mouse cerebral cortex that neurons are functionally coupled in local clusters by means of propagating network oscillations in the beta frequency range. In the newborn mouse, this…

Action PotentialsSensory systemBiologyReceptors N-Methyl-D-AspartateSynapseMiceSubplatemedicineBiological neural networkAnimalsReceptorNeuronsMultidisciplinaryGap junctionGap JunctionsSomatosensory CortexElectrophysiologyMice Inbred C57BLElectrophysiologymedicine.anatomical_structureBiochemistryAnimals NewbornCerebral cortexSynapsesNMDA receptorCarbacholNeuronCortical columnNeurosciencee-Neuroforum
researchProduct

Breakdown of Thalamo-Cortical Connectivity Precedes Spike Generation in Focal Epilepsies

2017

Electroencephalography (EEG) spikes and focal epileptic seizures are generated in circumscribed cerebral networks that have been insufficiently described. For precise time and spatial domain network characterization, we applied in patients with focal epilepsy dense array 256-channel EEG recordings with causal connectivity estimation by using time-resolved partial directed coherence and 3T-magnetic resonance imaging-derived cortical and thalamus integrity reconstruction. Before spike generation, significant theta and alpha bands driven information flows alterations were noted from both temporal and frontal lobes to the thalamus and from the thalamus to the frontal lobe. Medial dorsal and ven…

AdultMale0301 basic medicineThalamusAction PotentialsElectroencephalographySensitivity and Specificity03 medical and health sciencesEpilepsy0302 clinical medicineThalamusBiological ClocksNeural PathwaysConnectomemedicineHumansIn patientFocal EpilepsiesCerebral CortexDense arraymedicine.diagnostic_testGeneral NeuroscienceReproducibility of ResultsElectroencephalographymedicine.disease030104 developmental biologyThalamo corticalFrontal lobeFemaleEpilepsies PartialNerve NetPsychologyNeuroscience030217 neurology & neurosurgeryBrain Connectivity
researchProduct

The relative contribution to the plantar-flexor torque of the soleus motor units activated by the H reflex and M response in humans.

2000

Abstract This study proposes a method of quantifying the relative contribution to the plantar-flexor torque of soleus H and M responses evoked by tibial nerve stimulation. For ten subjects, the amplitude of the twitch produced by the H wave was plotted against the corresponding potential, for stimuli producing H without M (i.e. in the ascending portion of the H-recruitment curve). It was then assumed that the contribution of H to twitches produced by M plus H was similar for similar H waves on the curve-descending portion. Hence, the contribution of M was estimated, for the range of M waves including those accompanying H max . The estimated mechanical contributions of H and M wave increase …

AdultMaleAction PotentialsPlantar flexionH-ReflexNuclear magnetic resonanceTorqueHumansTibial nerveMuscle SkeletalSoleus musclePhysicsElectromyographyGeneral NeuroscienceAnatomyM responsemusculoskeletal systemElectric StimulationMotor unitAmplitudeMuscle Fibers Slow-TwitchTorqueMuscle Fibers Fast-TwitchH-reflexTibial NerveMuscle ContractionNeuroscience letters
researchProduct

Changes in cerebellar activation pattern during two successive sequences of saccades

2002

The changes in the cerebellar activation pattern of two successive fMRI scanning runs were determined for visually guided to‐and‐fro saccades in 12 healthy volunteers familiar with the study paradigm. Group and single subject‐analyses revealed a constant activation of the paramedian cerebellar vermis (uvula, tonsils, tuber, folium/declive), which reflects constant ocular motor activity in both runs. A significant decrease in activation of the cerebellar hemispheres found in the second run is best explained by either a decrease in attention or the effects of motor optimization and learning. The significant, systematic changes of the cerebellar activation pattern in two successive runs were n…

AdultMaleCerebellumOcular motorAction PotentialsStimulationFunctional LateralityActivation patternCerebellar CortexReaction TimeSaccadesmedicineHumansRadiology Nuclear Medicine and imagingFastigial nucleusBrain MappingRadiological and Ultrasound TechnologyVisually guidedEye movementOriginal ArticlesMiddle AgedMagnetic Resonance Imagingmedicine.anatomical_structureNeurologyCerebrovascular CirculationCerebellar vermisFemaleNeurology (clinical)AnatomyPsychologyNeurosciencePhotic StimulationPsychomotor PerformanceHuman Brain Mapping
researchProduct